Review



rabbit polyclonal igg anti pax6  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Proteintech rabbit polyclonal igg anti pax6
    Rabbit Polyclonal Igg Anti Pax6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg anti pax6/product/Proteintech
    Average 96 stars, based on 101 article reviews
    rabbit polyclonal igg anti pax6 - by Bioz Stars, 2026-03
    96/100 stars

    Images



    Similar Products

    polyclonal anti rabbit igg irdye 800cw  (LI-COR)
    99
    LI-COR polyclonal anti rabbit igg irdye 800cw
    Polyclonal Anti Rabbit Igg Irdye 800cw, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti rabbit igg irdye 800cw/product/LI-COR
    Average 99 stars, based on 1 article reviews
    polyclonal anti rabbit igg irdye 800cw - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    polyclonal ba 1000 ab 2313606 vector laboratories  (Vector Laboratories)
    96
    Vector Laboratories polyclonal ba 1000 ab 2313606 vector laboratories
    Polyclonal Ba 1000 Ab 2313606 Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal ba 1000 ab 2313606 vector laboratories/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    polyclonal ba 1000 ab 2313606 vector laboratories - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    rabbit polyclonal igg anti pax6  (Proteintech)
    96
    Proteintech rabbit polyclonal igg anti pax6
    Rabbit Polyclonal Igg Anti Pax6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg anti pax6/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal igg anti pax6 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    rabbit polyclonal igg to α tubulin  (Proteintech)
    96
    Proteintech rabbit polyclonal igg to α tubulin
    Rabbit Polyclonal Igg To α Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg to α tubulin/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal igg to α tubulin - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    rabbit polyclonal igg to stat3  (Proteintech)
    96
    Proteintech rabbit polyclonal igg to stat3
    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, <t>STAT3</t> and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.
    Rabbit Polyclonal Igg To Stat3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg to stat3/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal igg to stat3 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    rabbit igg control polyclonal antibody  (Proteintech)
    95
    Proteintech rabbit igg control polyclonal antibody
    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, <t>STAT3</t> and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.
    Rabbit Igg Control Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit igg control polyclonal antibody/product/Proteintech
    Average 95 stars, based on 1 article reviews
    rabbit igg control polyclonal antibody - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    rabbit polyclonal igg to tff3  (Proteintech)
    94
    Proteintech rabbit polyclonal igg to tff3
    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, <t>TFF3,</t> CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.
    Rabbit Polyclonal Igg To Tff3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg to tff3/product/Proteintech
    Average 94 stars, based on 1 article reviews
    rabbit polyclonal igg to tff3 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    goat anti rabbit igg polyclonal antibody  (LI-COR)
    99
    LI-COR goat anti rabbit igg polyclonal antibody
    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, <t>TFF3,</t> CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.
    Goat Anti Rabbit Igg Polyclonal Antibody, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg polyclonal antibody/product/LI-COR
    Average 99 stars, based on 1 article reviews
    goat anti rabbit igg polyclonal antibody - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    goat anti rabbit polyclonal antibody  (Boster Bio)
    96
    Boster Bio goat anti rabbit polyclonal antibody
    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, <t>TFF3,</t> CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.
    Goat Anti Rabbit Polyclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit polyclonal antibody/product/Boster Bio
    Average 96 stars, based on 1 article reviews
    goat anti rabbit polyclonal antibody - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    irdye 800cw goat polyclonal anti rabbit igg  (LI-COR)
    99
    LI-COR irdye 800cw goat polyclonal anti rabbit igg
    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, <t>TFF3,</t> CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.
    Irdye 800cw Goat Polyclonal Anti Rabbit Igg, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irdye 800cw goat polyclonal anti rabbit igg/product/LI-COR
    Average 99 stars, based on 1 article reviews
    irdye 800cw goat polyclonal anti rabbit igg - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

    Journal: Molecular Medicine Reports

    Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

    doi: 10.3892/mmr.2025.13783

    Figure Lengend Snippet: ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

    Article Snippet: Rabbit polyclonal IgG to STAT3 , Proteintech Group, Inc. , 10253-2-AP , 1:500.

    Techniques: Expressing, Knockdown, Western Blot, Control, Over Expression

    Comparison of the expression of inflammatory mediators (A) IL-1β, (B) IL-6 and (C) IL-8, as well as (D) STAT3 between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (E) IL-1β, (F) IL-6, (G) IL-8 and (H) STAT3 mRNA levels and ST3Gal1 expression. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. Data in A-D are presented as box and whisker plots, and those in E-H as scatter plots. Statistical significance for group comparisons in A-D was assessed using the unpaired t-test; correlation analyses in E-H were performed via Pearson's correlation coefficient. **P<0.01.

    Journal: Molecular Medicine Reports

    Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

    doi: 10.3892/mmr.2025.13783

    Figure Lengend Snippet: Comparison of the expression of inflammatory mediators (A) IL-1β, (B) IL-6 and (C) IL-8, as well as (D) STAT3 between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (E) IL-1β, (F) IL-6, (G) IL-8 and (H) STAT3 mRNA levels and ST3Gal1 expression. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. Data in A-D are presented as box and whisker plots, and those in E-H as scatter plots. Statistical significance for group comparisons in A-D was assessed using the unpaired t-test; correlation analyses in E-H were performed via Pearson's correlation coefficient. **P<0.01.

    Article Snippet: Rabbit polyclonal IgG to STAT3 , Proteintech Group, Inc. , 10253-2-AP , 1:500.

    Techniques: Comparison, Expressing, Gene Expression, Whisker Assay

    ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

    Journal: Molecular Medicine Reports

    Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

    doi: 10.3892/mmr.2025.13783

    Figure Lengend Snippet: ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

    Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

    Techniques: Expressing, Knockdown, Western Blot, Control, Over Expression

    Comparison of (A) MUC2, (B) TFF3 and (C) CDX2 mRNA levels between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (D) MUC2, (E) TFF3 and (F) CDX2 mRNA levels and ST3Gal1 expression. Data in A-C are presented as box and whisker plots, and those in D-F as scatter plots. Statistical significance for group comparisons in A-C was assessed using unpaired t-test. Correlation analyses in D-F were performed using Pearson's correlation coefficient. *P<0.05 and **P<0.01. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. *P<0.05 and **P<0.01. NS, not significant; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2.

    Journal: Molecular Medicine Reports

    Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

    doi: 10.3892/mmr.2025.13783

    Figure Lengend Snippet: Comparison of (A) MUC2, (B) TFF3 and (C) CDX2 mRNA levels between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (D) MUC2, (E) TFF3 and (F) CDX2 mRNA levels and ST3Gal1 expression. Data in A-C are presented as box and whisker plots, and those in D-F as scatter plots. Statistical significance for group comparisons in A-C was assessed using unpaired t-test. Correlation analyses in D-F were performed using Pearson's correlation coefficient. *P<0.05 and **P<0.01. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. *P<0.05 and **P<0.01. NS, not significant; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2.

    Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

    Techniques: Comparison, Expressing, Whisker Assay, Gene Expression